Journal: Antibiotics

Article Title: Wound-Healing Potential of Engineered Lysin GRC-ML07 in Pseudomonas aeruginosa Infected Wounds in Immunocompromised Mice

doi: 10.3390/antibiotics14121248

Figure Lengend Snippet: Activation of NF-κB ( a ) and IRF ( b ) signaling pathways in THP1-Dual™ monocytes by alginate gel components, GRC-ML07, and bacterial lysates. NC—negative control; PC—positive control. Dashed line indicates luciferase activity in water-treated cells. RLU—relative light units. MHB (NC)—Mueller–Hinton broth sterile; Alg—Sodium alginate, 1%; ML07—GRC-ML07; R848 (PC)—TLR7/8 ligand; Pam3CSK4 (PC)—Synthetic triacylated lipopeptide and a TLR2/TLR1 ligand; LPS (PC)—Purified lipopolysaccharide from E. coli strain 055:B5. TLR4 ligand; PG ab —Bacterial peptidoglycan. TLR2 ligand; CB FILTR—Filter-sterilized culture broth after intact P. aeruginosa cells cultivation; S/N ML07 —Supernatant of P. aeruginosa after the treatment with 1 mg/mL of GRC-ML07; S/N ML07 FILTR—Filter-sterilized supernatant of P. aeruginosa after the treatment with 1 mg/mL of GRC-ML07. All experimental variants were performed in triplicate in two independent experiments. ****— p < 0.0001 compared to MHB stimulated samples.

Article Snippet: 7 , R848 (PC) , TLR7/8 ligand (Invivogen) , Sterile.

Techniques: Activation Assay, Protein-Protein interactions, Negative Control, Positive Control, Luciferase, Activity Assay, Sterility, Purification